Characterization of particle-bound reactive oxygen species in tobacco smoke

JIAYUAN ZHAO (1), Philip K. Hopke (1), Gunter Oberdorster (2), Bob Gelein (2), Pamela Wade-Mercer (2), Irfan Rahman (2)

(1) Center for Air Resources Engineering and Science, Clarkson University, Potsdam, NY 13676, USA (2) Department of Environmental Medicine, University of Rochester, Rochester, NY 14642, USA

     Abstract Number: 666
     Last modified: May 14, 2010

Abstract
The aim of this study is to determine the amount of particle-bound ROS in tobacco smoke, using 2,7-dichlorodihydrofluorescein diacetate (DCFH) as the fluorescent probe. Both research cigarettes and real cigarettes were tested using mainstream and side stream generated by a standard smoking machine. The samples were collected on Teflon filters. The filters were leached with DCFH working solution and sonicated and incubated together in 15 minutes. The fluorescence of the samples were measured to determine the amount of particle-bound ROS and the results were provided with the unit of equivalence to H2O2. The results will be provided in amount of ROS/ cigarette and compared with background ROS amount in atmosphere of Rochester, NY.