AAAR 31st Annual Conference
October 8-12, 2012
Hyatt Regency Minneapolis
Minneapolis, Minnesota, USA
Abstract View
Applications of Real-Time Quantitative Polymerase Chain Reaction in Assessing the Pseudomonas Aeruginosa in Air Environment
MIAO-CHING CHI, Chang Gung University of Science and Technology, Taiwan
Abstract Number: 313 Working Group: Indoor Aerosols
Abstract Nosocomial infection has become a great public-health problem worldwide. Nosocomial infection in health care setting is among the major causes of and increased mortality among hospitalized patient. Because of the increased length of stay for infected patients, the economic costs are considerable. Therefore, the purpose of our current study was to build up the optimal conditions of real-time qPCR method to analyze Pseudomonas aeruginosa. Real-time qPCR was applied to evaluate the concentration of Pseudomonas aeruginosa in air environments. The result showed that the lowest detect limit of this method could be 5.7 copies/micro-liter, with a correlation coefficient value of 0.999. In evaluating the extraction time of bacteria form filter, there were the most cultural concentrations for shaking 1 min. Moreover, the activity and DNA of Pseudomonas aeruginosa storaged in low temperature would be damaged. Therefore, the results suggested that the samples should be immediately analyzed after air sampling. In air sampling, the concentrations of Pseudomonas aeruginosa in indoor air were from 2.34×10$^3 copies/m$^3 to 3.70×10$^3 copies/m$^3. This study indicated that Pseudomonas aeruginosa can exist in air environment. In the future, it is necessary to monitor the activity of Pseudomonas aeruginosa for estimating the route of transmission of nosocomial infection.