AAAR 31st Annual Conference
October 8-12, 2012
Hyatt Regency Minneapolis
Minneapolis, Minnesota, USA
Abstract View
Quantifying Ligand Adsorption to Nanoparticles using Tandem Differential Mobility – Mass Analysis
SUVAJYOTI GUHA, Xiaofei Ma, Michael Tarlov, Michael Zachariah, University of Maryland, College Park
Abstract Number: 318 Working Group: Instrumentation and Methods
Abstract Quantification of ligands adsorbed to different bionanoparticles is important in the context of drug delivery and therapeutics. Here we present a new approach for ligand quantification by combining differential mobility analyzer (DMA) with aerosol particle mass analyzer (APM). This approach is advantageous compared to other existing techniques because it can be applied to heterogeneous samples and is independent of bionanoparticle property, ligand morphology and conformation. We demonstrate this approach with two examples, adsorption of different concentrations of bovine serum albumin to gold nanoparticles and adsorption of rituxan, a monoclonal human antibody to polystyrene latex spheres at different pH. Our results appear to be in excellent agreement with literature. Thus we anticipate that this hyphenation of DMA with APM can become an important method for quantification of ligands in the near future.