AAAR 31st Annual Conference
October 8-12, 2012
Hyatt Regency Minneapolis
Minneapolis, Minnesota, USA
Abstract View
Physical and Chemical Properties of Iron Oxide Nanoparticles that Contribute to Cellular Toxicity and Acellular Production of Hydroxyl Radical
JESSICA CHARRIER, Christoph Vogel, Aamir Abid, Ian Kennedy, Cort Anastasio, University of California, Davis
Abstract Number: 371 Working Group: Health Related Aerosols
Abstract Engineered nanoparticles are increasingly used in consumer products causing more frequent human exposure to these materials. The potential health risks of nanomaterials – and, especially, the physical and chemical properties that increase toxicity - are not well understood. One likely mechanism of toxicity of nanoparticles is their ability to produce reactive oxygen species, such as hydrogen peroxide and hydroxyl radical (˙OH), which can induce oxidative stress, inflammation, and cell death. To investigate which properties of nanoparticles are associated with toxicity, we quantified accelular production of ˙OH and the upregulation of markers of inflammation and oxidative stress in macrophage cells from six iron oxide nanoparticle types. The iron oxide nanoparticles have varying physical properties (e.g., surface area and surface charge) and different ratios of iron oxidation state (Fe(0), Fe(II) and Fe(III)). We will report on the relationship between the chemical and physical properties of Fe nanoparticles, acellular ˙OH production, and upregulation of inflammatory and oxidative stress-related markers in macrophage cells. We will also discuss the relation between the cell-free ˙OH results and in-vitro cellular oxidative response.