AAAR 31st Annual Conference
October 8-12, 2012
Hyatt Regency Minneapolis
Minneapolis, Minnesota, USA
Abstract View
Monodisperse Poly(lactide–co–glycolic acid)–based Nanocarriers for Gene Transfection
JEONG HOON BYEON, Jeffrey Roberts, Department of Chemistry, Purdue University
Abstract Number: 63 Working Group: Nanoparticles and Materials Synthesis
Abstract This contribution describes a simple, aerosol–based method for fabricating monodisperse particles containing mixtures of poly(lactide–co–glycolic acid) [PLGA], protamine sulfate (Prot), and poly(L–lysine) [PLL] as nanocarriers for gene transfection. Aqueous solutions of PLGA, Prot, and PLL were collison–atomized, and the resulting aerosolized droplets were dried “on the fly” to form solid particles, which then were electrostatically size–classified into 50 nm, 100 nm, and 200 nm samples. Agarose gel retardation assay confirms that collected particles bind plasmid deoxyribonucleic acid (pDNA), and measurements of cell viability and transfection reveal that the fabricated nanocarriers have a lower cytotoxicity (>85% in cell viability) and a higher transfection efficiency (>8.7 × 10$^5 in relative light units, RLU, per mg) than does 25 kDa polyethyleneimine (~50% and 6.8 × 10$^5 RLU per mg). The strategy outlined in this work is potentially generalizable as a new platform for creating polymeric therapeutic nanocarriers using only clinically–approved starting materials in a single–pass configuration.