AAAR 31st Annual Conference
October 8-12, 2012
Hyatt Regency Minneapolis
Minneapolis, Minnesota, USA
Abstract View
Generation of Reactive Oxygen Species from Source-Oriented, Ambient Submicron Particulate Matter in a Cell-free Surrogate Lung Fluid Solution
NICOLE RICHARDS, Jessica Charrier, Keith Bein, Anthony Wexler, Cort Anastasio, UC Davis
Abstract Number: 717 Working Group: Health Related Aerosols
Abstract Exposure to air pollution particulate matter (PM) is correlated to adverse health effects, but the mechanisms for this effect are unclear. One proposed pathway for PM-mediated health effects is the generation of reactive oxygen species (ROS) such as superoxide radicals, hydrogen peroxide and hydroxyl radical, which can cause oxidative stress. While past studies have quantified ROS production from PM, there are no studies that correlate ROS measurements from different particle sources. To help address this issue, we collected six different source-oriented particle mixtures (e.g., cooking, vehicular, residential heating, and biomass emissions), segregated into quasi-ultrafine (≤ 170 nm) and submicron (170 – 1000 nm) size fractions, in Fresno, California during summer 2008 and winter 2009. Hydroxyl radical and hydrogen peroxide formation were quantified by HPLC from the source-oriented particles in a cell-free surrogate lung fluid containing four antioxidants (ascorbate, citrate, glutathione, and uric acid). The oxidative potential of the particles was also measured by the dithiothreitol (DTT) method. Preliminary results show that different source-oriented PM samples have different rates of hydroxyl radical formation and DTT consumption. In these studies we found, on average, no correlation between particle size and either OH formation or oxidative potential.