American Association for Aerosol Research - Abstract Submission

AAAR 34th Annual Conference
October 12 - October 16, 2015
Hyatt Regency
Minneapolis, Minnesota, USA

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Diversity and Difference of Bacteria in PM2.5 before and during the APEC Meeting Days in Beijing City

PENGRUI DU, Rui Du, Zedong Lu, Pingqing Fu, University of Chinese Academy of Science

     Abstract Number: 98
     Working Group: Bioaerosols

Abstract
Particulate matters with aerodynamic diameter <2.5µm (PM2.5) have been a significant air pollutant in Beijing city. Bacteria in PM2.5 are thought to be the important cause for various allergies and respiratory diseases. People feel uncomfortable and the number of patients increases when the air quality condition is poorer, especially during the haze weather processes. However, the differences of bacterial communities in PM2.5 under different air quality conditions have not been reported. In the present study, we collected PM2.5 samples from15 Oct to 12 Nov. Due to the government’s strong control about the air quality, APEC blue sky appeared during the meeting opened days. The weather conditions of sampling days were divided into three levels according to the Air Quality Index (AQI) (AQI=0-100, 101-200, 201-300) and analyzed the dissimilarities of bacteria between them through the 16s rRNA gene high-throughput sequencing using the Ilumina Miseq PE250 platform. A total of 429 different kinds of bacteria in genus level were detected and Sphingomonas (7.94%), Paracoccus (6.60%), kocuria (5.13%), Rubellimicrobium (3.86) and Arthrobacter (3.18%) were the primary groups in the 80 thousand gene sequences. The majority of the inhalable microorganisms were nonpathogenic to human. Similarities of bacterial communities in different samples were more than 60% and no obvious significant differences were discovered either good or poor air qualities. The results suggest that during the severe haze pollution episodes, the role of bacteria in PM2.5 to human health should be reconsidered.