AAAR 34th Annual Conference
October 12 - October 16, 2015
Hyatt Regency
Minneapolis, Minnesota, USA
Abstract View
Examining the Physicochemical Properties of Silver Nanoparticles in Simple and Complex Simulated Gastric Fluid
JESSICA AXSON, Diana Stark, Amy Bondy, Justin Keeney, Sonja Capracotta, Andrew Maynard, Martin Philbert, Ingrid Bergin, Andrew Ault, University of Michigan, Ann Arbor MI
Abstract Number: 99 Working Group: Nanoparticles and Materials Synthesis
Abstract Silver nanoparticles (AgNPs) are present in a wide variety of commercial products and can enter the human body through oral exposure. Examining the fundamental physicochemical changes in nanoparticle size under conditions representative of the gastrointestinal (GI) tract is important for elucidating the impacts of AgNP on the human body. This work examines the kinetics of AgNP in simple (NaCl + HCl) simulated gastric fluid (SGF) as a function of pH (SGF at pH 2, 3.5, 4.5 and 5), size (20 nm and 110 nm AgNPs), and coating (Citrate and PVP). Rapid AgNP growth was observed on 30 second timescales using Nanoparticle Tracking Analysis (NTA), which is explained by the generation of Ag+ in acidic environments that precipitates with Cl-, leading to particle growth and facilitating particle aggregation by decreasing their repulsion in solution. Additionally we explore the use of Surface Enhanced Raman microspectroscopy (SERs) as a useful tool for examining protein corona formation on AgNP in more complex (NaCl+ HCl+ pepsin) SGF as proteins with in the human body can bind to the AgNP surface potentially altering their function within the body. This study indicates a need to further understand the importance of initial size, physicochemical properties, and kinetics of AgNPs after ingestion to assess their potential toxicity.