AAAR 35th Annual Conference October 17 - October 21, 2016 Oregon Convention Center Portland, Oregon, USA
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Examining Nanoparticle Oral Exposure Using a Multi-Vessel Gastrointestinal Apparatus
JESSICA AXSON, Andrew Ault, Justin Keeney, Sun Chenxi, Ingrid Bergin, Martin Philbert, Andrew Maynard, University of Michigan, Ann Arbor MI
Abstract Number: 451 Working Group: Aerosol Exposure
Abstract Nanoparticles (NPs) are present in a wide variety of commercial products and industrial processes. One way NPs can enter the human body is through oral exposure. Examining the fundamental physicochemical changes in NP size under conditions representative of the gastrointestinal (GI) tract is important for elucidating the impacts of NPs on the human body. To examine the change in NP characteristics as they are digested in vitro, a well-known and well-characterized pharmaceutical drug dissolution testing apparatus, the USP II, was utilized. Prior NP studies have rotated or stirred the media with in heated baths, with subsequent additions of media representing that from gastrointestinal compartments. In this work, a three vessel USP II apparatus was designed to mimic conditions and movement within the gastrointestinal system. Using this approach we will be able to monitor the simultaneous movement and changes to NP as they go from one gastrointestinal compartment to the next, in the stomach, duodenum, and jejunum. In this study, we examined 20 nm citrate coated silver nanoparticles (AgNP), Ni-NP, and CoO-NP within the USP II multivessel system (MVS). Particles were monitored in situ for changes in diameter using nanoparticle tracking analysis (NTA) and separately for changes in surface structure, including the formation of a protein corona, using scanning electron microscopy with electron diffraction x-ray spectroscopy (SEM-EDX). This research is supported through NIH Grant U01ES020128.