AAAR 35th Annual Conference October 17 - October 21, 2016 Oregon Convention Center Portland, Oregon, USA
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Fine Variability of Pollens, Fungal Spores and Bacteria during Spring 2016 in Cyprus Island
DOMINIQUE BAISNEE, Roland Sarda-Esteve, Francois Fenaille, Minh Thang Le, Jean Sciare, Iasonas Stavroulas, Michel Thibaudon, Marc Peyraut, Christophe Bossuet, CEA
Abstract Number: 673 Working Group: Bioaerosols
Abstract In the frame of Bio Chemical Collectors (BCC) research project from the French Atomic Energy Commission (CEA), we have deployed a new experimental procedure for the collection and the analysis of atmospheric Bioaerosols on membrane filters without culture. This experimental procedure has been applied during the spring 2016 in Cyprus Island from March 29 to April 28, at the Cyprus Atmospheric Observatory (CAO) at Agia Marina Xyliatou. For the collection and detection of pollen and fungal spores every two hours we used a spore trap Hirst Type (LANZONI, It). For the collection, detection and analysis of bacteria every 2 hours we used a sequential sampler (LECKEL, 47/50) at a flow of 2.3m3/h. To evaluate the presence of bacteria in the Particulate Matter below 1 micron (PM1) we used 47 mm Sartorius Gelatine Filter with a pore size of 3µm. The samples were cultivated on 3 separate and specific substrates for the identification of bacterial species. For the total bacteria in the Particulate Matter below 10 microns (PM10) in we used 47 mm Nuclepore membrane with a pore size of 2µm. The analysis of total bacteria has been done by a new analytical method developed within this research program. This method consists in the detection of Muramic Acid which is a specific tracer of bacteria by Liquide Chromatography coupled with a High Resolution Mass Spectrometer (LC HS MS/MS). The final study has been done in cooperation with the Cyprus institute for the characterization of atmospheric ice nucleating particle (INP) in the frame of the EU-funded project : Impact of Biogenic versus Anthropogenic emissions on Clouds and Climate: towards a Holistic UnderStanding (BACCHUS).
This work is supported by the CBRN-E R&D research program from Joint ministerial program of R&D against CBRN-E threats.