AAAR 36th Annual Conference October 16 - October 20, 2017 Raleigh Convention Center Raleigh, North Carolina, USA
Abstract View
Use of Online Yeast Sensor (SLEPTor) for Analyzing PM2.5 Effects on Autophagy Gene Expression
TING ZHANG, Maosheng Yao, Peking University
Abstract Number: 695 Working Group: Bioaerosols
Abstract Previously, in our lab we have developed a yeast based on PM2.5 toxicity sensor that is able to real-time analyze yeast responses to PM2.5 exposure. Here, we further applied the method to study the effects of PM2.5 exposure on the expressions of an autophagy gene of the yeast in real-time when exposed to the air samples. Our preliminary data suggest that the protocol is working and the system sensitivity need to be further improved to obtain a quicker response. In this presentation, a detailed protocol will be presented and relevant data will be also shown. Our work presents a very different solution for studying health effects of PM2.5 than currently available ones.