10th International Aerosol Conference September 2 - September 7, 2018 America's Center Convention Complex St. Louis, Missouri, USA
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Toxicity Evaluation of Secondary Organic Aerosol on Human Lung Cells
PRATITI HOME CHOWDHURY, Tanya Lasitza Male, Quanfu He, Michal Pardo, Yinon Rudich, Weizmann Institute of Science
Abstract Number: 1240 Working Group: Aerosols and Health - Connecting the Dots
Abstract Adverse health effects due to exposure to particulate matter (PM) are among the most important global environmental health risks. However, the effects of exposure to secondary organic aerosols (SOA), major component of the global aerosols, are largely unknown. To understand the effect of real environmental exposure on the respiratory system we simulated SOA from biogenic and anthropogenic sources mimicking day time ambient aerosol. In this study, we exposed lung epithelial cells (A549) to aged SOA particles (equivalent to 3 days) using air liquid interface (ALI) cell exposure system, thus mimicking the deposition of fine particles in the lung. SOA were generated using Oxidation Flow Reactor (OFR) that provides a highly oxidizing environment that simulates atmospheric oxidation processes under NOx-free and high NOx conditions. Both anthropogenic and biogenic volatile organic compounds (naphthalene and α-pinene, respectively) were used as the SOA precursors. Our preliminary results show a significant decrease in cell viability and significant increase in ROS when exposed to SOA in compared to clean air and incubator control. other parameters related to cell oxidative stress and inflammation were shown to vary after exposure to SOA. This study elucidates at least in part some of the biological responses of SOA and their biochemical pathway of toxicity.