Abstract Number: 626 Working Group: Aerosol Toxicology
Abstract The heavy metals and organic compounds adsorbed on the surface of PM may cause pro-inflammatory effects by production of reactive oxygen species (ROS) leading to respiratory, and cardiovascular illnesses. The PM2.5 concentration in metro cities of India has crossed the alarming levels. Limited studies have reported the link of PM concentration with respiratory parameters observed with spirometer while none has reported the toxicological effect of PM on human lung cells. Therefore, this study will present the cytotoxicity, ROS and omics of PM on A549 human lung cell. The personal exposure mass was collected on five stages (PM10-PM2.5, PM2.5-PM1.0, PM1.0-PM0.5 PM0.5-PM0.25 and after filter) of cascade impactor (SKC, USA) at road site near main gate of Indian Institute of Technology Delhi during January to June 2017. The 6-hrs average mass concentration of PM collected on all stages was varying on monthly basis with lowest concentration was observed in March (122.2±76.5 µg/m3) and highest (203±74.64µg/m3) in January. PM2.5 were also collected at urban background (15 m height) away from the roads and any other source activity. The average mass concentration of background PM was highest in January 2017 (221.9±98.6 µg/m3) and lowest in March (84.2±17.8µg/m3). The large variation in PM mass and its toxic components is expected to cause very different effect on lung cell viability. Therefore, this study will discuss the monthly variability in PM mass concentration and its effect on viability of human lung cell (A549). The effect of personal exposure mass of coarse (PM10-PM2.5), fine (PM2.5-PM1.0, PM1.0-PM0.5 PM0.5-PM0.25) and ultrafine (After filter) particles will be presented. The lung cells exposed to 3-days composites particulate mass will be studied for cytotoxicity, ROS and omics. The concentration for both the personal and ambient were found to be more than NAAQS (National Ambient Air Quality Standards) which is set as 60 µg/m3 for 24 hours and 40 µg/m3 annually so this study stood essential and was performed for the first time in any metro-cities in India.