10th International Aerosol Conference September 2 - September 7, 2018 America's Center Convention Complex St. Louis, Missouri, USA
Abstract View
Bacterial and Fungal Aerosol Emissions from Different Land Types
XINYUE LI, Maosheng Yao, Peking University
Abstract Number: 669 Working Group: Bioaerosols
Abstract Human activities are changing the land-use types and patterns continuously, and also affecting the interaction between land and atmosphere in the fields of physics, chemistry and biology. As a significant source of atmospheric bioaerosols, microorganisms from land emission have noteworthy influence on regional ambient air quality, public health and climate change. However, the studies about the characteristics and variations of microbial aerosols in various types of land-use are still not sufficient. Here we report a study of microbial aerosols in several different land-use areas in an urban setting including garden, forest, lakes, wet land, farmland, livestock farm, sewage treatment plant, main roads, smeltery and bare land. Samples collected with a new high flow portable biological aerosol sampler (HighBioTrap) developed by our group. Plate cultivation and real-time quantitative PCR were respectively used to quantitatively analyze the concentrations of culturable microorganisms and total microorganisms. The characteristics of microbial community structures in different areas were also analyzed with high throughput gene sequencing: 16s rRNA for bacteria and ITS for fungi. Our results showed that there were differences in their concentration and compositions of microbial aerosols among different land-use types. The results showed that the livestock farm had the highest bacterial aerosol concentration levels, followed by main roads, smeltery, sewage treatment plant, lakes, garden, forest, bare land, farmland and wet land. The rates of bacteria and fungi aerosols (B/F) suggested microbial community structures varied from 21.7 (in forest) to 0.8 (upon bare land). Sequencing results showed there were significant differences in their microbial aerosol structures. For fungi community, the Tremellales and Pleosporales were detected to dominate in most sites, but a large proportion of fungi was unclassified. This work researched the features of microbial aerosols from multifarious land-use types more comprehensively, found significant diversity of microbial aerosols among different types of land-use. It provided more valuable views to identify the ambient air quality and evaluate population health risks exposure to urban micro-bioaerosol.