AAAR 37th Annual Conference October 14 - October 18, 2019 Oregon Convention Center Portland, Oregon, USA
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Continuous and Efficient Virus Sampling and Enrichment System
HYEONG RAE KIM, Sanggwon An, Jungho Hwang, Yonsei university
Abstract Number: 383 Working Group: Bioaerosols
Abstract Against the shortcomings of long-time consuming sampling or incubation of bioaerosols, enrichment technologies have recently been proposed using microfluidic chip. However, microfluidic chip has a limitation for high air sampling flow rate (up to only a few mL/min). Therefore, microfluidic chip is not realizable for air sampler itself in the field requiring high sampling flow rate for the real-time bioaerosols monitoring.
A possible alternative could be to exploit an air sampler, sampling the bioaerosols and simultaneously enriching them in the sampler continuously. However, commercial air samplers are batch-type samplers which cannot continuously deliver the liquid bioaerosols sample to the sensors connected after air sampler for real-time monitoring. Also, air-to-liquid enrichment rate of these samplers for the single bacteria size (0.8 µm) was as low as 600 to 43,000 min-1. So far, there is no system for continuous sampling and enrichment of airborne virus which exist in air at low concentrations. Herein we report a continuous and efficient bioaerosol sampling and enrichment system which can be utilized not only for airborne bacteria but also virus particles. For these purposes, an air sampler was developed and optimized for efficient bioaerosols sampling. Concanavalin A (Con A) coated magnetic particles (CMPs) were applied for enriching the bioaerosols.
Bioaerosol capturing performance was evaluated using APS and SMPS. Bacteria (S.aureus) and influenza A virus (H1N1) capturing efficiency were 80% and 70.3%, respectively with an air flow rate of 10 lpm and an applied voltage of -7 kV. Enrichment capability of our system was evaluated using PCR device. PCR cycle number was advanced as 5.3 after virus enrichment. The highly enriched bioaerosol sample compensates for low collection efficiency of air samplers and can also allow to monitor bioaerosols rapidly by significantly reducing the bioaerosols sampling or culturing time.