AAAR 37th Annual Conference October 14 - October 18, 2019 Oregon Convention Center Portland, Oregon, USA
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Antibiotic Resistance Genes Distribution Analysis in Common Respiratory Pathogens
MINFEI WANG, Maosheng Yao, Peking University
Abstract Number: 826 Working Group: Bioaerosols
Abstract Abstracts:Respiratory infection affects millions of lives worldwide every year. The transmissions of airborne pathogens and antibiotic resistance genes (ARGs) make the situation even worse. Certain bacterial pathogens such as Pseudomonas aeruginosa, Haemophilus influenza and Methicillin-resistant Stphylococcus aureus (MRSA) can be exhaled out from the infected patients. On the other hand, antibiotic resistance genes (ARGs) in ambient particulate matters have emerged as an increasing global health concern. However, the resistance mechanisms of respiratory pathogens remain to be incompletely understood, which may aggravate the misuse of antibiotics. In this study, the levels of 39 ARG subtypes resistant to seven common classes antibiotics such as quinolones, β-lactams, macrolides, tetracyclines, sulfonamides, aminoglycosides and vancomycins and two mobile genetic elements (MGE) including tnpA encoding transposase and intI1 encoding integrase classI in P.aeruginosa, H. influenzae and MRSA were screened. The antimicrobial susceptibility test was conducted to further explore the expression of ARG subtypes detected in P. aeruginosa. The results indicated that the Quinolone resistance gene qepA?99.90%??the Tetracycline gene tetC (99.97%) and the Macrolide resistance gene ermC?70.14%?were the most abundant ARG subtypes in P. aeruginosa, H. influenza and MRSA, respectively. Overall, here we intended to profile the ARG subtypes distribution and to explore the ARGs expression levels in several common respiratory pathogens, which could be useful for the antibiotic therapy guidance and might alleviate the social panic of ARGs in ambient environment.