AAAR 37th Annual Conference October 14 - October 18, 2019 Oregon Convention Center Portland, Oregon, USA
Abstract View
Exposure to Portable Gasoline Generator Emissions and Its Effects on Renal Function and Lung Histology using Rat Model
GODSON ANA, Emmanuel Obansa, University of Ibadan
Abstract Number: 838 Working Group: From Aerosol Dosimetry and Toxicology to Health
Abstract Previous studies had examined gasoline generator emissions (GGEs) profile but information about their sub-acute and non-fatal toxicological effects is inadequate. This study assessed the levels of GGEs and their effects on renal indices and lung histopathological conditions.
Three experimental groups of eight rats each were subjected to 5, 10 and 15 minutes exposure to GGEs per day for twenty-one days between the hours of 4 and 5pm with the fourth group not being exposed. Levels of CO, NO2, VOC and PM2.5 in the emission chamber were recorded simultaneously using the appropriate air samplers and compared with guideline limits. Twelve hours after completion of exposure monitoring, blood was collected for renal function tests via ocular puncture while lung tissues were collected after the animals were sacrificed. Hematoxylene and Eosin (H & E) method was used to examine the lung tissues. Data collected were analysed using ANOVA and statistical significance was set at p= 0.05.
One-hour TWA levels of CO, NO2, VOC and PM2.5 recorded in the chamber were 968ppm, 2.43ppm, 100ppm and 17.6 mg/m3 respectively. Blood urea nitrogen and creatinine tests showed significant (p < 0.05) increases in the test groups compared with controls. Histopathological examinations showed lesions like alveolar edema, infiltration of inflammatory cells and thickening of the alveolar interstitium in lung tissues of the exposed rats.
Exposure to GGEs at non-lethal concentrations chronically portends deleterious effects on renal and pulmonary systems. There is a need to minimise exposure and to embrace cleaner fuels.