AAAR 32nd Annual Conference
September 30 - October 4, 2013
Oregon Convention Center
Portland, Oregon, USA
Abstract View
Use of Air-Sampling-Culturing, Free Settling and Filtration Revealed Strikingly Different Bacterial Aerosol Species through High Throughput Gene Sequence
MINGZHEN LI, Kai Wei, Yunhao Zheng, Jing Li, Zhuanglei Zou, Maosheng Yao, Xu Zhencheng, Peking University
Abstract Number: 626 Working Group: Bioaerosols: Characterization and Environmental Impact
Abstract Bacterial types in the air are important in many aspects including health and climate. In this work, we employed Andersen impactor-sampling-culturing, free settling and filtration to study the bacterial aerosol species in both indoor and outdoor environments with different climatic conditions. The experiments were conducted in 4 outdoor and 2 indoor environments in Guangzhou and Haikou, China. High throughput gene sequence was performed for CFU samples pooled together from culturing and free settling, respectively, over one week and for one filter sample (24 hours at a flow rate of 100 L/min) collected on a particular day during the week.
Our preliminary data showed that regardless of environments use of high volume filtration only obtained about half bacterial species types as compared to those obtained by culturing and free settling methods. For outdoor environments, it seems that culturing and free settling performed equally well in most cases with respect to the bacterial species profiling, while culturing turned to perform better than free settling in indoor environments. Our statistical analysis at 97% confidence level also indicated that use of culturing and free settling also resulted in higher values of Shannon index (bacterial diversity) and Ace (bacterial richness). In this work, we have detected more than 1000 bacterial species in both indoor and outdoor environments, and these two environments were found to exhibit different distribution patterns in addition to different species present in respective environments. Contrary to traditional belief, this study showed that filtration sample would yield less bacterial species than culturing methods employed here through high throughput gene sequence. In addition, our data also suggest that indoor and outdoor environments have their different bacterial emission sources.
Key words: Bacterial species, Culturing, Filtration, Indoor, Outdoor and High throughput gene sequence