AAAR 32nd Annual Conference
September 30 - October 4, 2013
Oregon Convention Center
Portland, Oregon, USA
Abstract View
Lung Cancer Inhibitory Effect of PLGA-coated Budesonide and Polyphenon E in A/J Mice
JINGJIE ZHANG, Virginia Commonwealth Univerisity
Abstract Number: 705 Working Group: Health Related Aerosols
Abstract Polymer nanoparticles as drug carriers have potential advantage in drug-delivery and controlled-release. A properly-selected polymer shell not only improves the solubility and stability of drugs in aqueous solutions, but also reduces the drug toxicity. Aerosol delivery is a promising approach for drug delivery, especially for the diseases in the respiratory tract and the lung. Aerosolized drugs in sub- micrometer-sizes can be inhaled and delivered directly into the lung, thus enhancing the local-regional drug level and reducing the exposure to surrounding organs other than the lung. In this study, we evaluated the advantage of delivering encapsulated drugs via aerosol administration. Two anticancer drugs, budesonide (hydrophilic) and polyphenon E (hydrophobic) were chosen in this experiment. These two drugs have been shown to inhibit benzo[a]pyrene (B[a]P) - induced lung tumorigenesis in A/J mice. PLGA and PVP were used as the polymer coating to encapsulate the drugs separately. Coated-drugs were prepared by the emulsion-evaporation technique. Excess solvent was washed out. Resulted drug-loaded particles were collected by the centrifugal separation and stored in a refrigerator. Prior to the use as-prepared particles were dispersed into an aqueous suspension (with pH 2.0) by ultrasonification. The suspension of drug-loaded particles was atomized using a custom-built Collison atomizer. B[a]P-induced A/J mice were treated three times per week, for 20 weeks consecutively before they were euthanized. Their lungs were examined under a dissection microscope. The tumor number and tumor size were scored and recorded. Our result shows that PLGA-coated budesonide and polyphenon E had inhibitory effects on lung tumors. The detail of this work will be presented in this poster.