Abstract Bioaerosols are airborne biological particles present in different environments, composed of plant and animal material. The biological diversity of swine barn air is well known, often composed of entire microorganisms such as Lactobacillus, Clostridium, Mycobacterium, Pasteurella and Streptococcus. The microbial concentration in the air may reach 10$^9 bacteria per m$^3 of air. Many pathogens are part of airborne bioaerosol community in these establishments. The goal of this study is to understand the behaviour in bioaerosols of Streptococcus suis, a swine pathogen causing meningitis, septicemia, endocarditis, pneumonia and also a zoonosis agent implicated in three human epidemics in China. There are 35 serotypes of S. suis based on the immunogenic reaction of their capsule. Serotype 2 is the most often isolated in case of swine and human diseases. Our hypothesis is that certain serotypes could be preferentially aerosolized and thus, more likely to be inhaled and reach the airways. To verify this hypothesis, a stainless steel aerosol chamber with multiple sampling ports has been designed and built. To mimic natural aerosolization processes, bubbling has been used to nebulize the S. suis suspensions. S. suis bioaerosols have been recovered on polycarbonate filters (0.4 micro-meter). qPCR and PMA-qPCR technologies were used to distinguish intact from damaged cells in the samples and thus, the resistance of serotypes to aerosolization processes. During aerosolization, serotype 2 was preferentially aerosolized compared to serotype 5 and its ratio was enriched in the air when compared to the nebulizing solution. Also, the serotype 2 membrane was less damaged than the serotype 5 after the aerosolization. The preferential aerosolization phenomenon is not explained yet but the capsular composition could allow an easiest aerosolization and a protection against the stresses induced thus conserving its infectious state.