AAAR 33rd Annual Conference
October 20 - October 24, 2014
Rosen Shingle Creek
Orlando, Florida, USA
Abstract View
Physico-chemical qualification and refinements of a new portable bio aerosols collector: BIODOSI
ROLAND SARDA-ESTEVE, Jean-Maxime Roux, CEA
Abstract Number: 16 Working Group: Bioaerosols
Abstract As part of the overall security program for the fight against the spread of toxic biological agents (CBRN-E) the CEA (Center of Atomic Energy) has developed a portable collector based on electrostatic precipitation: BIODOSI. This new type of particle collector is lightweight (500g), silent (no pump) and can collect the particulate matter (PM) during more than 8 hours at a flow rate up to 7 LPM. The on board electronic can control and adjust all the collection parameters to maintain the collection efficiency at is optimum value. The first version BV1 (Sarda Esteve et al. AAAR, 2012; J.M Roux et al. ISPC-BWA 2013) was tested in real atmospherics condition at the super site ACTRIS, LSCE SIRTA-5, France in July 2013 to determine the capability of this new potable collector to capture low levels of fungal spores in the atmosphere. The collection efficiency (0.3 µm to 20 µm) was monitored on line. The samples were extracted in 10 ml of pure MilliQ water by sonication and the liquid extracts were compared to filter based traditional sampling methodology. All the liquid extracts were measured by Ion Chromatography (IC) for the major ions, IC-Pulsed Amperometric Detection (PAD) for the sugars and Liquid Chromatography Mass Spectrometry (LC-MS) for the specific analysis of Manitol and Arabitol (Sarda Esteve et al. AAAR, 2013). We report here the results of the physico-chemical characterization in real atmosphere for the first prototype BIODOSI Version 1 (BV1) and the refinements on the last prototype BV3 (Fig.1). The results obtained by measuring chemicals tracers of fungal spores as Manitol and Arabitol (Bauer et al 2008) show that this new portable collector can be used to identify bio aerosols events (Sarda Esteve and Roux, IOP, 2013).
This work was supported by the CBRN-E R&D research program from CEA.