AAAR 33rd Annual Conference
October 20 - October 24, 2014
Rosen Shingle Creek
Orlando, Florida, USA
Abstract View
Simultaneous Real-time Fluorescence and Microscopy Measurements of Bioaerosols during the BIODETECT 2014 Campaign in Paris Area
DOMINIQUE BAISNEE, Michel Thibaudon, Raphaelle Baumier, Gavin McMeeking, Greg Kok, David O'Connor, John Sodeau, J. Alex Huffman, Walfried Lassar, Kyle Pierce, Martin Gallagher, Ian Crawford, Georges Salines, Roland Sarda-Esteve, CEA
Abstract Number: 569 Working Group: Bioaerosols
Abstract During the BIODETECT 2014 intensive campaign which was conducted at CEA/LSCE SUPERSITE (suburban) and LHVP Paris (urban), simultaneous measurements of particulate matter below 10 micron in size (PM10) were made using real-time fluorescence and microscopy-based methods. One of the objectives of the BIODETECT 2014 intensive campaign at these two sites was to evaluate the ability of a Wide Band Integrated Bioaerosol Sensor (WIBS) to detect bioaerosol events in suburban/urban sites impacted frequently by pollution events. The microscopic identification of bioaerosols was done by the National Network of Survey for Airborne contaminants (RNSA) to identify pollens and fungal spores. This network has more than 25 years of experience in the fast detection and identification of the species present in the atmosphere using a pollen/fungal spores trap. The multiple WIBS instruments used here were inter-compared and calibrated at CEA. We present here the experimental strategy that we used to constrain the WIBS fluorescence measurements and the preliminary results obtained from the one month experiment. One important result is that the WIBS can track bioaerosols events including pollen and fungal spores even when air masses are heavily impacted by traffic emissions.
More work is needed to do a source apportionment of fluorescent contributors and to define which co-located measurements are needed to constrain the LIF method for the improved detection of bioaerosols.
This work is supported by the CBRN-E R&D research program from CEA/DAM