AAAR 36th Annual Conference October 16 - October 20, 2017 Raleigh Convention Center Raleigh, North Carolina, USA
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Toxicological and Chemical Properties of Fine Particles Produced from Various Sources
MINHAN PARK, HungSoo Joo, Kwangyul Lee, Tsatsral Batmunkh, Lucille Joanna Borlaza, Heung-Bin Lim, Han-Jae Shin, Myoseon Jang, Ji Yi Lee, Min-Suk Bae, Kyu-Hyuck Chung, Daeun Kim, Kihong Park, Gwangju Institute of Science and Technology
Abstract Number: 478 Working Group: Health Related Aerosols
Abstract Fine particles in the ambient atmosphere are a complex mixture of various chemical components. Determination of source-specific toxicity can be an useful way to disentangle effects of PM on human health. The goal of this study is to assess variability in toxicity of particles produced from various sources, diesel and gasoline engine, biomass burning, coal burning, and dust, including secondary organic aerosols. Physical and chemical characterization of aerosols was conducted to relate source-specific toxicity of aerosols to their chemical components. In addition, ambient PM samples collected from urban, rural, roadside, and industrial areas were analyzed for determination of their chemical composition and toxicity. Chemical characteristics such as ion, metal, organic carbon (OC)/elemental carbon (EC) was determined by ion chromatography (IC), inductively coupled plasma mass spectrometry (ICP-MS), and thermal-optical transmittance (TOT) method, respectively. OC speciation was conducted by gas chromatography-mass spectrometry (GC-MS). Toxicity test was conducted using chemical and biological assays. For chemical assay, oxidative potential (OP) was measured by OPDTT and OPESR. For biological assay, various end points, cytotoxicity, mutagenicity, oxidative stress, and inflammation, were determined by neutral red uptake (NRU) assay and water soluble tetrazolium salt (WST-1), Ames test and comet assay, DCF-DA (2’,7’-dichlorofluorescein diacetate) assay, IL-6 and IL-8, respectively. A statistical analysis was applied to relate chemical and toxicity of various aerosols. Preliminary results show the rankings of source-specific risks based on the results obtained up to the present can be suggested; 1) Primary aerosols from various sources: DEP > biomass burning particles > coal burning particles (550°C) > Tunnel dust, 2) Secondary organic aerosols: Toluene SOA > Isoprene SOA > TMB SOA > α-pinene SOA, and 3) Ambient aerosols (PM2.5): Roadside > Industrial > Urban > Rural site.