AAAR 36th Annual Conference October 16 - October 20, 2017 Raleigh Convention Center Raleigh, North Carolina, USA
Abstract View
Development of A High Volume Portable Bioaerosol Concentrating Sampler
HAOXUAN CHEN, Maosheng Yao, Peking University
Abstract Number: 692 Working Group: Bioaerosols
Abstract Bioaerosols exposure can cause many adverse health effects even death when deadly pathogens involved. Existing bioaerosol samplers either have low sampling flow rate or have higher impaction stress (resulting in low concentration or injuries to the cells) such that further analysis of the samplers is often is a great challenge. Here, we have developed a portable high volume bioaerosol sampler (1200 L/min) that was powered using a battery and optimized to have an impaction velocity of around 10 m/s. The sampler was shown to not only have relatively higher physical collection efficiencies of ambient air particles with a cutoff size of 2 µm, but also resulted in greater diversity of bacterial cells compared to the Andersen type sampler as revealed from gene sequence analysis. In addition, the HighBioTrap sampler was also shown to be able to concentrate large volume of air into small amount of liquids at a rate of about 2 millions/min. The collected air samples can be easily analyzed using loop-mediated isothermal amplification (LAMP) for low level pathogen detection.