AAAR 36th Annual Conference October 16 - October 20, 2017 Raleigh Convention Center Raleigh, North Carolina, USA
Abstract View
Use of Automobile Filter Samples for Profiling Global Bioaerosols
JING LI, Maosheng Yao, Peking University
Abstract Number: 694 Working Group: Bioaerosols
Abstract Bioaerosols in theory are all generated from the ground and could vary greatly across locations. In this work, we employed a previously established automobile filter method to obtain air samples from 15 cities of 10 countries under 8 different climate zones. Total 117 auto filter samples were obtained. Using culturing, qPCR, ELISA and also gene sequence method, we have analyzed biological contents from these auto filter samples. Our data showed that some filter dusts contained about 104 CFU/mg bacteria and 103 CFU/mg fungi with a total bacterial count of up to 106 cells/mg. The dust samples were also shown to contain various allergens up to 10 ng/mg. Gene sequence data revealed there were remarkable differences in bacterial structures from different countries. Preliminary data also showed that bioaerosol size distributions also varied greatly across locations. Using the developed protocol, we are able to easily profile global bioaerosol characteristics and thus accelerate the understanding of airborne micorbiota.