10th International Aerosol Conference September 2 - September 7, 2018 America's Center Convention Complex St. Louis, Missouri, USA
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Effects of Temperature and Water Condensation on the Sampling of Infectious Influenza H1N1 Virus Aerosol through Water-based Condensational Growth
MAOHUA PAN, Leah Carol, Aneal Mamane, John Lednicky, Arantzazu Eiguren Fernandez, Susanne Hering, Hugh Fan, Chang Yu Wu, University of Florida
Abstract Number: 1254 Working Group: Bioaerosols
Abstract Inhalation exposure to airborne influenza viruses can pose major health risks to humans and animals, and potentially result in significant economic losses. A 2017 study reported that up to 646,000 people in the word die every year of influenza. However, studies about sampling of airborne infectious influenza viruses are limited due to inefficiency of existing virus aerosol samplers, and the sampling conditions vary a lot considerably, making sampling results difficult to compare to each other. In prior studies, we demonstrated high collection efficiency for airborne infectious influenza H1N1 viruses by the VIable Virus Aerosol Sampler (VIVAS). The objective of this study was to evaluate the effects of temperature and amount of water condensed onto the collection liquid of SESI (Super-Efficient Sampler for Infectious virus, an improved version of the VIVAS) on the viability of the collected infectious influenza H1N1 viruses.
In controlled laboratory experiments, H1N1 influenza virus (80-120 nm) was aerosolized using a Bioaerosol Nebulizing Generator (BANG) and the aerosolized virus was collected by the SESI. This SESI is composed of four components: Conditioner, Initiator, Moderator, and Collector. Sampling temperatures (10, 20, and 33ºC) were pre-set for the Collector, whereas the moderator’s temperature was adjusted accordingly to keep the accumulated collection liquid to minimum (< 0.1ml). During tests of the effects of volume of accumulated condensed water on virus viability, the temperature of the collector was kept at 10 ºC whereas the Moderator’s temperature was changed from 6 to 14 ºC, which resulted in different amounts of accumulated water in the collection media. PBS with 0.5% w/v BSA Fraction V was used as the collection liquid, and Madin Darby canine kidney (MDCK) cells using a standard median tissue culture infectious dose (TCID50) assay was used to determine the infectious virus titer (#/mL) of Influenza H1N1 virus.
When the sampling period was 20 min, survivability of the influenza virus was higher when the collector temperature was 10 ˚C or 33 ˚C than at 20 ˚C. Further analysis is needed to explain this reproducible finding. The amount of water condensed onto the collection liquid during the 20 min sampling did not have significant effects on the viability of the infectious viruses, which suggest that this parameter need not to be taken into consideration for sampling infectious influenza viruses within 20 min. This is the first time the effects of sampling temperature and the amount of water condensed onto the collection liquid on the viability of infectious viruses are evaluated using a SESI.