Abstract Number: 186 Working Group: Aerosol Chemistry
Abstract Numerous epidemiological studies have shown adverse health effects by fine and coarse aerosol particles such as PM2.5 and yellow sand. For this reason, aerosol cell exposure studies are currently required. However, cell exposure studies have some problems when we use aerosol particles collected by a filter sampling. For example, it is difficult to collect sufficient amount of aerosol particles for exposure, influence of contaminants derived from filter is inevitable at the time of exposure and operation to obtain particles from filter is complicated. Therefore, in this study we developed a high-volume sampler using virtual impactor and cyclone techniques for collecting aerosol in powder form without using a filter. This sampler can collect about 100 mg of coarse particles and fine particles separately in 2-3 weeks of operation. We collected fine and coarse aerosol particles at Keio University (in Yokohama) with this instrument. Chemical analysis of the collected particles has been carried out followed by DTT (Dithiothreitol) Assay to measure the oxidizing ability which is an indicator of the in-vivo oxidative stress of aerosol particles.