10th International Aerosol Conference September 2 - September 7, 2018 America's Center Convention Complex St. Louis, Missouri, USA
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Rapid Bioaerosol Exposure Risk Analysis in Typical Indoor Environments
MINFEI WANG, Haoxuan Chen, Maosheng Yao, Peking University
Abstract Number: 656 Working Group: Bioaerosols
Abstract Exposure to bioaerosols can lead to adverse health effects, such as respiratory disorders, allergic diseases and toxic reactions. Some certain highly-infectious agents can pose a deadly threat to health even in a very low dose. Accordingly, it is of significant importance for sampling infectious bioaerosols with low concentration. In this study, a high flow portable bioaerosol sampler (HighBioTrap) with a sampling flow rate of 1200 L/min was used to collect air samples in three different indoor environments (subway station, hospital and student dormitory). The air samples were concentrated into small amount of mineral oil and further analyzed using real-time quantitative polymerase chain reaction (qPCR), culture, loop-mediated isothermal amplification (LAMP) and high-throughput gene sequencing technology. The results indicated that the HighBioTrap can concentrate bioaerosols efficiently. Among three typical environments, total bacterial aerosols was detected up to 6.23×105/m3 in the hospital air samples. According to the gene sequencing analysis, subway air samples were observed to have the greatest diversity and richness of both bacterial and fungal community. Some differences were also observed among the top five genera of bacteria of all air samples collected from different environments. Using the LAMP method, we did not detect common human respiratory pathogens in the air samples collected, possibly due to lower levels. Overall, here we have developed a protocol for rapid microbial detection in a typical environment, by integrating a portable high volume sampler along with LAMP method. In the future, this method can be further used in various indoor environments for rapid pathogen detection.