10th International Aerosol Conference September 2 - September 7, 2018 America's Center Convention Complex St. Louis, Missouri, USA
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Protein Aggregation Kinetics by Electrospray Differential Mobility Analysis
KALEB DUELGE, Vince Hackley, Michael Zachariah, University of Maryland, College Park
Abstract Number: 911 Working Group: Aerosol Chemistry
Abstract Protein aggregation is an important concern in protein therapeutics, resulting in adverse immunological response in patients. New therapeutics seeking FDA approval require some form of an induced aggregation study to indicate the stability of the formulation. Techniques to investigate aggregation include: fluorescence, analytical ultracentrifugation and light scattering with or without an initial separation step. Size exclusion chromatography and field flow fractionation separate the protein particles by size in solution and then detect the particles using light absorbance or scattering. These techniques allow for the calculation of aggregation kinetics by monitoring the loss of the protein monomer with time. We present results on the use of Electrospray Differential Mobility Analysis (ES-DMA) as an alternative means to study protein aggregation. ES-DMA is used to determine the temporal distribution of monomer, dimer and higher aggregates. These results are used to determine rate laws and rate constants under various thermal and buffer conditions. Comparison to more traditional methods will be examined.