AAAR 37th Annual Conference October 14 - October 18, 2019 Oregon Convention Center Portland, Oregon, USA
Abstract View
How Realistic Are In-Vitro Doses Used for Particle Toxicity Studies: Is There Something “Wrong” with Our In-Vitro Assays?
OTMAR SCHMID, Helmholtz Zentrum München
Abstract Number: 83 Working Group: From Aerosol Dosimetry and Toxicology to Health
Abstract There is an apparent gap between onset doses of inhaled particle toxicity observed in in-vitro cell and in-vivo animal models of the lung. Typically in-vitro assays are much less sensitive than animal models when considering tissue-delivered dose. I will show that many cell cultures assays do not even respond after receiving equivalent lifetime doses for realistic exposure scenarios.
In this talk a strategy for direct comparison of in-vitro and in-vivo dose-response curves will be presented, typical onset doses as reported by in-vitro and in-vivo studies with soot and engineered nanoparticles will be compared and several hypothesis for the apparently low sensitivity of cell culture models will be discussed. This will provide insight into future perspectives of in-vitro particle toxicity studies and their relevance for assessing health risks due to exposure to inhaled particles.