Measuring Reactive Oxygen Species (ROS) Production of Particulate Matter through Air-Blood Barrier Array Module
SEONGBIN JO, Hannah Viola, Liang-Hsin Chen, Taekyu Joo, Shuichi Takayama, Nga Lee Ng,
Georgia Institute of Technology Abstract Number: 348
Working Group: Health-Related Aerosols
AbstractExpsoure to particulate matter (PM) causes a significant impact on human health. Many toxicological studies have examined various biological endpoints to study the adverse effects of PM exposure linked to respiratory diseases. However, their physiological relevance to human health and the inhibitory concentrations remain unclear. Furthermore, the extraordinary diversity in chemical properties and components of different ambient PM samples would lead to varied cellular responses from the PM exposure. Here, we introduce a newly-developed high-throughput air-blood barrier array (ABBA) module, which mimics the human lungs epithelium and endothelium interface that is capable of examining multiple cellular responses from exposure to PM samples. The air-blood barrier between the air-side epithelium and the blood-side endothelium provides a realistic model of the alveoli and respiratory bronchioles. The barrier strengthened with the co-cultured epi/endothelial cells establishes decreased permeability and induces PM deposition in a similar way to that of the alveoli cells. We use the ABBA module to investigate PM-induced reactive oxygen species (ROS) production, which can initiate inflammatory cascades and lead to oxidative stress, thus contributing to chronic lung diseases. Various assay parameters (dosage, sample incubation time, trans-epithelial electrical resistance, etc.) are controlled prior to the exposure experiments. The capability of the ABBA is demonstrated via measurements of ROS production of the co-cultured cells upon exposure to ambient PM samples collected in urban Atlanta. Dose response results will also be presented and discussed. The high-throughput ABBA module demonstrates the replicability and multiplicity for evaluating cellular responses upon aerosol exposure.