Assessing the Infectious Potential of Accidentally Produced Aerosols in Biological Laboratories

ASHLEY R. RAVNHOLDT, Danielle N. Rivera, Daniel N. Ackerman, Gabriel A. Lucero, Elizabeth A. Klug, Shanna A. Ratnesar-Shumate, Joshua L. Santarpia, University of Nebraska Medical Center

     Abstract Number: 489
     Working Group: Bioaerosols

Abstract
Laboratory-acquired infections (LAIs) continue to pose a significant risk to laboratory workers, largely due to bioaerosols produced during routine procedures and accidents. Building on previous research, this study investigates the infectious potential of these aerosols by utilizing the nonenveloped viral surrogate, MS2 bacteriophage. The choice of MS2 bacteriophage allows testing of whether culturable microorganisms can be transmitted through the aerosols quantified in preliminary fluorescent tracer studies. A specialized chamber equipped with a cascade impactor, an optical particle counter, and a separate filter facilitated the assessment of aerosol production during selected routine procedures and simulated mishaps. The samples were analyzed using quantitative PCR and plaque assays to determine the presence and viability of the aerosols captured. This research enhances understanding bioaerosol dynamics within laboratory environments, potentially contributing to improved safety protocols and protective measures for laboratory personnel.