AAAR 34th Annual Conference
October 12 - October 16, 2015
Hyatt Regency
Minneapolis, Minnesota, USA
Abstract View
Biotransformation of Various Saccharides and Production of Exopolymeric Substances (EPSs) by Cloud-borne Bacillus SP. 3B6
ANNE-MARIE DELORT, Maria Matulova, Slavomira Husarova, Martine Sancelme, Peter Capek, Université Clermont Auvergne, France
Abstract Number: 43 Working Group: Bioaerosols
Abstract The ability of Bacillus sp. 3B6, a bacterial strain isolated from cloud waters, to biotransform saccharides present in the atmosphere was evaluated using in situ 1D and 2D NMR spectroscopy. Bacillus is one of a genus most frequently described in the air and atmospheric waters. Sugars present in these environments have a biogenic origin; they include alditols, monosaccharides, disaccharides, oligosaccharides, and polysaccharides.
Bacillus sp. 3B6 was able to efficiently metabolize sugars, which could thus provide sources of energy for this bacterium and allow it to live and to be metabolically active in warm clouds. In addition, a number of these saccharides (L-arabitol, D-fructose, sucrose, D-glucose, cellotetraose, cellulose, and starch) were transformed to EPSs (ExoPolymeric Substances). We have clearly identified the structure of two EPSs as 1,6-alpha-galactan and partially acetylated polyethylene glycol (AcPEG). 1,6-alpha-galactan is a newly described polymer. The production of EPSs might protect this bacterium under hostile cloud environment conditions, including low nutrient availability, cold temperature and freeze-thaw processes, UV and radical exposure, and evaporation–condensation processes and thus to desiccation and osmolarity changes. EPSs could also have a potential role in atmospheric processes because they can be considered as secondary organic aerosols and efficient cloud condensation nuclei (1).
Acnowledgement:
This research has been supported by the CNRS LEFE-CHAT program, the French-Slovak Štefánik project No 17947UE, the French Government Scholarship (fellowship for S. Husárová).
Reference:
(1) Mária Matulová, Slavomíra Husárová, Peter Capek, Martine Sancelme and Anne-Marie Delort. Biotransformation of various saccharides and production of exopolymeric substances by cloud-borne Bacillus sp. 3B6. Environmental Science and Technology, 2014, 48, 14238−14247.