AAAR 34th Annual Conference
October 12 - October 16, 2015
Hyatt Regency
Minneapolis, Minnesota, USA
Abstract View
Resistant Microorganisms Present in Cattle Feedlots in Airborne in Tijuana, Mexico
LILIA HURTADO, Guillermo Rodriguez, Javier Emmanuel Castillo-Quiñones, Luisa Molina, Penelope Quintana, Jonathan Lopez, Marvic Carrillo, Universidad Autonoma de Baja California, Tijuana, Mexico
Abstract Number: 534 Working Group: Bioaerosols
Abstract Cattle industries are an important source of particulates and bioaerosols material. These substances have a detrimental effect on both public health and the environment. In Mexico the use of drugs in animal production has been an unregulated; following practice favors the inappropriate use of drugs causing the development of antimicrobial resistant strains of pathogenic and non-pathogenic bacteria. Feedlots were ideal place to study antibiotic resistance because bacteria are exposed to high levels antibiotics.
Air samples were taken with the M Air T (Millipore) at different distances from the center of activity at each site. Cultivation methods based on the viable counts of mesophilic bacteria, total and fecal coliforms, enterococci and other pathogenic bacteria. During air sampling, temperature, solar radiation, relative humidity and wind speed were measured.
The determination of antibiotic resistance was performed as follows instruction for CLSI 2015. The classification of strains was conducted according to data from the disc manufacturer (BBL).
The results of the studies on the resistance to antibiotics showed that the bacteria isolated were in quite a significant percentage resistant to antibiotics. In the case of penicillin, the sensitivity to 50% in the case of bacteria isolated.
The bacteria resistant to erythromycin were observed in 63%. A highest number of strains resistant to streptomycin (58%).
The number of strains resistant to tetracycline varied from 9% to 29%.
The number of strains resistant to vancomycin varied from 4% to 23%. A high proportion of antibiotic-resistant bacteria suggest that these strains had contact with antibiotics in the past and have acquired resistance to them or the resistance genes were transferred to them from other antibiotic-resistant bacteria. The highest number of resistant strains was selected if penicillin was used. This finding may be related to a long-term application of this antibiotic in medicine.