AAAR 34th Annual Conference
October 12 - October 16, 2015
Hyatt Regency
Minneapolis, Minnesota, USA
Abstract View
The Influence of Particulate and Gas-Phase Pollutants on Markers of Acute Airway Oxidative Stress in Active Adolescents
ROBY GREENWALD, Shiwei Gao, Georgia State University
Abstract Number: 539 Working Group: Health Related Aerosols
Abstract The Study of Air Pollution and Physical Activity investigates the influence of physical activity on air pollution exposure and consequent health effects in adolescents. High school students who participate in extra curricular athletic activities were recruited from two high schools in the Atlanta area, one near a major freeway and the other in a suburban area distant from roadways and other sources. Participants performed a suite of health outcome measurements before and after sports practice, including measurement of lung function and exhaled nitric oxide (eNO), and collection of exhaled breath condensate (EBC) samples. The concentration of both reduced and oxidized glutathione (GSH, GSSG) was measured in EBC, and %GSSG, the percent ratio of GSSG/(GSH+GSSG), was used as a marker of airway anti-oxidant capacity and acute oxidative stress. Air pollution exposure and inhaled dose was measured on site during sports practice and included PM2.5 mass, black carbon, particle number concentration (PNC) as well as ozone and other gas phase pollutants. Linear mixed models (both single and multi-pollutant) were used to assess the association between pollutant exposure and the change (post- compared to pre-practice) in eNO and %GSSG. In the case of eNO, no significant association was observed for any exposure variable. This could be due to the fact that post-exposure outcome measurements occurred immediately following practice while previous studies have suggested that eNO peaks approximately 1-2 hours post-exposure. Changes in %GSSG are expected to be observed immediately following exposure, and in the case of %GSSG, a significant association was observed for ozone and PNC exposure, but not for other particulate pollutants. Analysis of pollutant inhaled dose is ongoing, but at the present time, study results suggest that ozone has a more pronounced influence on acute airway oxidative stress than particulate pollutants in this panel of active adolescents.