AAAR 34th Annual Conference
October 12 - October 16, 2015
Hyatt Regency
Minneapolis, Minnesota, USA
Abstract View
Spectral Intensity Bioaerosol Sensor (SIBS): Description and Initial Characterization of a Novel Commercial Instrument for Spectrally-Resolved Fluorescence Measurements of Individual Particles
NICOLE SAVAGE, Tobias Könemann, Gary Granger, Gavin McMeeking, Ulrich Poeschl, Christopher Pöhlker, J. Alex Huffman, University of Denver, CO
Abstract Number: 699 Working Group: Bioaerosols
Abstract Until recently the understanding of bioaerosol properties had been limited by the lack of instrumentation capable of characterizing biological particles with high time and size resolution. The Wideband Integrated Bioaerosol Sensor (WIBS) developed by the University of Hertfordshire and recently licensed by Droplet Measurement Technologies (DMT) provides information about fluorescence of individual particles in two excitation and two emission channels. The Spectral Intensity Bioaerosol Sensor (SIBS) is a new instrument developed by DMT that improves upon the spectral resolution of the WIBS and other commercial bioaerosol sensors by providing higher resolution spectral information. The SIBS measures time-resolved fluorescence following sequential excitation at 280 and 370 nm over 16 emission channels spanning a range of approximately 300-720 nm. The instrument provides single particle fluorescence measurements, fluorescence lifetime, as well as particle size for each interrogated particle.
We performed an initial instrument comparison between the SIBS, WIBS, and Ultraviolet Aerodynamic Particle Sizer (UVAPS) instruments using polystyrene and bioparticle standards. Initial sizing and fluorescence spectral properties reported by the SIBS and other instruments will be reported.