AAAR 35th Annual Conference October 17 - October 21, 2016 Oregon Convention Center Portland, Oregon, USA
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Prediction of Delivery of SOA to Air-Liquid Interface Cells in Vitro Using Electrostatic Precipitator
ZECHEN YU, Myoseon Jang, Huanhuan Jiang, Tara Sabo-Attwood, Sarah Robinson, University of Florida
Abstract Number: 201 Working Group: Aerosol Exposure
Abstract In vitro studies using human airway lung cells have been frequently exploited to reduce animal use for the hazard screening of potential pulmonary toxic aerosols and augment understanding of cellular biological mechanisms in small quantities of aerosol. In particular, air-liquid interface (ALI) cell exposure method has been used to mimic the exposure of airway epithelial in pulmonary system to air pollutants. In order to better characterize the cellular responses of in vitro human airway cells to atmospheric organic aerosols, both efficient collection of aerosols and accurate dose prediction are essential. In this study, SOA was generated via ozonolysis of alpha-pinene and d-limonene using an indoor smog chamber. The produced SOA was efficiently delivered to ALI cells by a commercialized air-liquid exposure device (CULTEX RFS Compact) that was connected to an electrostatic precipitation (ESP). The in vitro ALI cells are cultured on the insert membrane and hosted by the exposure device. The SOA dose model was established based on several physical parameters, i.e. particle size, the deposition voltage, sampling flow rate and particle loss to the sampling lines. The feasibility of dose model was demonstrated against the filter mass of terpene-ozone SOA (R2=0.93). A clear dose-response relationship was obtained between SOA dose and the oxidative potential, which was measured using dithiothreithol assay. The dose-response relationship between toluene SOA and cellular responses (IL-8) was observed using the CULTEX RFS Compact ALI cell exposure system. The dose model integrated with exposure system are suitable for applying to toxic study of SOA.