Abstract Airborne transmission of pig pathogens such as the Porcine reproductive and respiratory syndrome virus (PRRSv) may be reduced in filtered buildings according to some field studies. However, the effectiveness against viruses and bacteria of filter systems need to be studied in an environmentally controlled and standardized environment. The objective of the study is to evaluate in an ASHRAE Standard 52.2-2012 test duct the capture efficiency of five combinations of MERV rated pre-filter and filter for Influenza virus, PRRSv and Streptococcus suis serotype 2 models. A Fluidized Bed Aerosol Generator (Model 3400A, TSI) and a 24-jet Collison Nebulizer (BGI) will be used conjointly or not to aerosolize Arizona road dust (ISO 12103-1, A2 Fine Test Dust, Powder Technology Inc), phages Phi6 (Influenza virus and PRRSv model), and Streptococcus thermophilus (Streptococcus suis serotype 2 model). Agglomeration of airborne particles coming from the two aerosol generators will take place in a mixing chamber that will be connected to the aerosol injection line of the test duct. Airborne particles will be counted by connecting two Optical Particle Sizers (sampling rate: 1L/min, Model 3330, TSI) both upstream and downstream of the tested filter system. AGI-30 impingers (sampling rate: 12.5L/min, All Glass Incorporated) will be used to sample the airborne virus and bacteria in 20mL of buffer. Samples taken from upstream and downstream of the tested filter system will be analyzed by culture and PCR to quantify viable/infectious and total microbial models, respectively. The herein study highlights the challenges of artificially reproducing the size distribution of environmental airborne particles and its effect on the capture efficiency of filters against virus and bacteria as well as using microbial models in a non-biocontained working space.