AAAR 35th Annual Conference October 17 - October 21, 2016 Oregon Convention Center Portland, Oregon, USA
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The Application of Real-Time Monitoring Techniques to Estimate the Inhaled Dose of Volatile and Semivolatile Organic Toxicants from Vaping e-Cigarettes
MARIELLE BRINKMAN, Sydney Gordon, Stephanie S. Buehler, Samera Hamad, Courtney A. Granville, Battelle Public Health Center for Tobacco Research
Abstract Number: 478 Working Group: Electronic Cigarettes - Particle Generation
Abstract Although machine smoking using an established puffing regimen can provide information on the identity of toxic emissions, measuring human toxicant exposures from the use of electronic cigarettes requires the incorporation of actual human vaping behaviors. We are applying human puff topography and real-time and batch sample mass spectrometry-based techniques to characterize harmful and potentially harmful constituent (HPHC) exposures from vaping e-cigarettes. The real-time techniques used in our laboratory for the analysis of mainstream smoke and exhaled breath are proton transfer reaction-mass spectrometry (PTR-MS) for continuous puff-by-puff characterization of selected volatile HPHCs, and an electrical low pressure impactor for the characterization and collection of fine and ultrafine particles. The collected particulate allows us to use batch sample mass spectrometry techniques to characterize the semi-volatile HPHCs that deposit in the body as a function of particle size. Real-time techniques offer the advantage of immediate analysis of dynamically changing e-cigarette emissions in order to more closely approximate user’s real-world HPHC exposures, and the ability to make measurements in exhaled breath over time to determine rates of volatile HPHC clearance from the body. Batch sample mass spectrometry-based techniques, including two-dimensional gas chromatography–time-of-flight mass spectrometry (GC×GC-TOFMS), allow us to discover new chemical emissions in addition to detect part-per-trillion levels of established HPHCs. Taken as a whole, these techniques can be used to generate some of the evidence needed to determine where a given tobacco product lies on the continuum of harm in the delivery of toxicants to the user. Such techniques can also be applied to determine the effect of variation in an e-cigarette product’s operating parameters and e-liquid content on human exposures.