AAAR 35th Annual Conference October 17 - October 21, 2016 Oregon Convention Center Portland, Oregon, USA
Abstract View
Single Living yEast PM2.5 Toxicity Sensor (SLEPTor) System
Kai Wei, Minghao Qiu, Rongfei Zhang, Liantong Zhou, Ting Zhang, Chunxiong Luo, MAOSHENG YAO, Peking University
Abstract Number: 496 Working Group: Health Related Aerosols
Abstract Air pollution has become one of major environmental challenges facing mankind in this century. However, current atmospheric toxicity studies are mostly offline with low sensitivity and poor understanding of biological responses. Here, we proposed an online analysis system dubbed as SLEPTor (Single Living yEast PM2.5 Toxicity Sensor) by integrating automated air sampling, microfluidics, GFP-tagged Saccharomyces cerevisiae yeast sensor as well as multiplexing automated fluorescence imaging system that allows us to study various protein responses of biological cells in vivo. In testing the SLEPTor, 63 yeast proteins were screened simultaneously here for their responses to both man-made ROS and also Beijing air samples. For the man-made, MRS3, MRS4, BSD2 and ZRC1 proteins were detected in increased abundances, among which the BSD2 expression, related to oxidative stress, was more pronounced, followed by MRS4 (for DNA repair) with a peak expression 20 min later. In contrast, HSP60 (oxidative stress), SSA1 (DNA repair) and MSB1 (MAPK pathway) proteins were observed to have elevated expressions visualized with time-lapsed movies when challenged with Beijing air samples 100 min later. Using the SLEPTor developed, we have observed sequential protein expressions for oxidative stress and DNA repair on the protein level in a single living cell. The same experiments were repeated twice using the SLEPTor and further confirmed by a spectra method. Among the proteins, SSA1 was shown to be sensitive to ambient air. The developed technology pioneers a new arena for investigating atmospheric health in an interactive manner with a large range of different functional proteins involved